Protein-Lipid interactions and Phospholipase Activity in Model Bio-membranes: Phospholipases are enzymes that catalyze hydrolysis of phospholipids in cell membranes. This is referred to as lipolytic or enzymatic activity. The products of hydrolysis are involved in normal physiological as well as pathophysiological cell functions including cell signaling, inflammation, allergy, apoptosis and tumorigenesis. Our research aims to elucidate the mechanism by which membrane physicochemical properties regulate lipolytic activity. This will provide important information toward understanding and eventually controlling these biological events in health and disease.
Experiments include measurements of lipolytic activity in vesicle model membranes as well as biophysical characterization of the vesicles. Quantitative models are being developed for the functional relation between membrane structure and enzymatic activity.