Gpcr HOt SpoT (GHOST) Residues
(Based on GRoSS Alignment using Ballesteros-Weinstein Numbering Scheme)
Native ACtivation HOt spot (NACHO) Residues
(Residues that change structural contacts during activation)
TM1: 1.53
TM2: 2.43
TM3: 3.43, 3.46, 3.50
TM5: 5.55, 5.58, 5.62
TM6: 6.36, 6.37, 6.40, 6.41
TM7: 7.49, 7.53, 7.54
Conserved inter-HelIcal Contact (CHICO) Residues
(Residues that define the GPCR Structural Fold, as the receptors couple to the same set of G proteins and arrestins)
TM1: 1.43, 1.46, 1.50, 1.53, 1.54, 1.57
TM2: 2.42, 2.43, 2.44, 2.45, 2.46, 2.47, 2.50, 2.51, 2.53, 2.54, 2.58
TM3: 3.34, 3.35, 3.36, 3.37, 3.38, 3.39, 3.40, 3.41, 3.42, 3.43, 3.45, 3.46, 3.47, 3.51
TM4: 4.46, 4.49, 4.50, 4.53, 4.57
TM5: 5.47, 5.57, 5.60, 5.61
TM6: 6.44, 6.47, 6.48, 6.51, 6.52
TM7: 7.38, 7.39, 7.41, 7.42, 7.45, 7.46, 7.47, 7.50, 7.53
Common to both NACHO and CHICO Lists
TM1: 1.53
TM2: 2.43
TM3: 3.43, 3.46
TM7: 7.53