AP BIOLOGY OUTLINE FOR CELLULAR BIOLOGY: CELLS: 1. Historical Development of the Cell Concept 2. Cell Structure and Function A. Techniques of biochemistry, centrifugation, and microscopy (light and electron) in understanding cells B. Structure and Function of Cell Organelles C. The passage of materials across cell membrane 3. Comparison of Prokaryotic and Eukaryotic Cells 4. Viruses and their relationship with cells CELL DIVISION: 1. Structure of Chromosomes 2. Mitosis and Meiosis in Plants and Animals 3. Cytokinesis in Plants and Animals; Control of Cell Division ESSAY QUESTIONS: 1959: Some of the differentiated structures of plant and animal cells are cell walls, plasma membranes, chromosomes, chloroplasts, mitochondria, and spindle fibers. Discuss four of these with respect to: 1) function and 2) physico-chemical nature 1960: Discuss each of the following, writing a paragraph or two for each one: a) the structure and role of the cell membrane b) the formation of cell walls in plant cells c) the structure and role of chloroplasts d) the structure and role of mitochondria 1963: a. Make a schematic diagram of a generalized plant or animal cell, showing the structure of its parts as revealed by electronmicroscopy. Make a diagram the size of a full page and label it completely, indicating whether the cell is from a plant or an animal. b. List the parts included in your diagram and describe briefly the activities or functions thought to be performed by each one. 1964: a. Describe the structure of the cell membrane as revealed by electronmicroscopy and iochemical studies. b. Explain how the passage of substances through the cell membrane is regulated by the physical and chemical properties of the substances involved. c. Explain how the concentration of a solute on either side of a semi-permeable membrane affects osmosis. 1965: Biologists and biochemists have made outstanding progress within the past quarter century in elucidating principles and structures which govern the activities of living matter. These areas of progress include: A. The structure and code of the DNA molecule B. The use of radioactive isotopes as tracers in biological processes C. The citric acid cycle and its relationship to mitochondria *D. The use of electron microscopy in revealing the structure of the cell Discuss any one of these developments and its impact on biological thought and progress. Your answer should include: a. a brief account of the development b. the names of the most prominent investigators involved c. the nature of its impact on biology 1969: Suppose a team of scientists is examining the cells of a newly discovered species. They observe under the light microscope an organelle that appears to be different from any that has been described before. Assume that you are director of the research team. Describe the methods that you would have the team use to determine whether the struc- ture is a mitochondrion, ribosome, lysosome, nucleolus, or indeed a new organelle. Discuss the advantages and limitations of each method in revealing the role of the unknown organelle in the living cell. 1970: Electronmicroscope studies have revealed the probable structure of plasma membranes and the membranes of various cell components. a. Describe the kinds of observations and experiments that are used to study the basic structure and molecular components of these membranes. b. Discuss mechanisms by which materials are thought to move across membranes. c. Discuss the significance of membranes in the biochemical events which occur in mitochondria and chloroplasts. 1975: All living cells exploit their environment for energy and for molecular components in order to maintain their internal environments. Describe the roles of several different membrane systems in these activities. 1978: Describe a model of the cell membrane of a eukaryotic cell and discuss different ways in which substances move across the membrane. 1981: Describe the structural arrangement and function of the membranes associated with each of the following eukaryotic organelles: a. Mitochondrion b. Endoplasmic Reticulum c. Chloroplast d. Golgi Apparatus 1983: Describe the fluid-mosaic model of a plasma membrane. Discuss the role of the membrane in the movement of materials through by each of the following processes. a. Active Transport b. Passive Transport 1984: Describe the structure of a generalized eukaryotic plant cell. Indicate the ways in which a nonphotosynthetic prokaryotic cell would differ in structure from this generalized eukaryotic plant cell. 1987: Discuss the process of cell division in animals. Include a description of mitosis and cytokinesis, and of the other phases of the cell cycle. Do not include meiosis. 1992: A laboratory assistant prepared solutions of 0.8 M, 0.6 M, 0.4 M, and 0.2 M sucrose, but forgot to label them. After realizing the error, the assistant randomly labeled the flasks containing these four unknown solutions as flask A, flask B, flask C, and flask D. Design an experiment, based on the principles of diffusion and osmosis, that the assistant could use to determine which of the flasks contains each of the four unknown solutions. Include in your answer (a) a description of how you would set up and perform the experiment; (b) the results you would expect from your experiment; and (c) an explanation of those results based on the principles involved. (Be sure to clearly state the principles addressed in your discussion.) 1993: Membranes are important structural features of cells. (a) Describe how membrane structure is related to the transport of materials across a membrane. (b) Describe the role of membranes in the synthesis of ATP in either cellular respiration or photosynthesis. 1994: Discuss how cellular structures, including the plasma membrane, specialized endoplasmic reticulum, cytoskeletal elements, and mitochondria, function together in the contraction of skeletal muscle cells.