Biology 315

Lecture

Principles of Microbiology

M. L. Summers, Ph.D.
Department of Biology
California State University Northridge


brightfield view of akinetes
epifluorescence view of akinetes

Two different views of the cyanobacterium Nostoc punctiforme bearing a transcriptional reporter plasmid containing a promoterless GFP gene driven by the promoter for avaK (a known akinete reporter gene).


Upper pannel; brightfield view of  plasmid bearing strain showing filamentous vegetative cells and larger akinete cells.

Lower pannel; epifluorescence view of GFP (green fluorescent protein) expression unique to akinetes.  This demonstrates  transcription of avaK in akinetes of this strain.

Textbook and Clicker:
   Microbiology: An Evolving Science. 2009.  JL Slonczewski and JW Foster (WW Norton publisher) ISBN: 978-0-393-97857-5
   The book may be purchased as textbook (bookstore) or purchased as an ebook (whole book or by the chapter) online at
http://nortonebooks.com/disciplines/Discipline.asp?DiscId=4

   A "clicker" is required for the class to earn your quiz points and can be purchased from a student previously taking this class, or from the website below.  A more expensive version from this company (Turning Technologies) is also available in the campus bookstore.  If you obtain another brand of clicker, it will not be compatible with our software. Be sure to order as soon as possible as we will start using them the third week of class.  Purchase the "ResponseCard RF Keypad" from Turning Technologies as described in the syllabus.

Syllabus:
  For Biol 315 lecture - Spring 2011

Lecture Notes:  are available here in PDF format; print at home prior to coming to class.

Lecture 1 and 2 - Intro and Microbial Life: Origin and Discovery
Lectures 3/4 - Part 1- Bacterial Cell Structure and Function
Lectures 4/5 - Part 2- Bacterial Cell Structure and Function
Lecture 6  - Archael and Eucakyal Cell Structure and Function
Lectures 7, 8, and 9 - Microbial Growth, and Control
Lectures 10 and 11 - Viruses
Exam 1 average was 79.3%
Lectures 13 and 14 - Energy, Enzymes and Catabolic Pathways 
Lectures 15 and 16 Respiration, Lithotrophy and Photolysis, Biosynthesis  
Lecture 17 Food and Industrial Microbiology 
Lectures 18-23 - Microbial Ecology: Symbioses and Nutrient Cycling  
Exam 2 average was 73.6%
Lectures 25-27 - Immunology   
Lectures 28-29 - DNA, Transcription, and Translation
Lectures 30-31 - Gene Regulation

Exam 3 study questions

Exam 3 in Thursday May 12 - the last day of class

the Final Exam is Tuesday May 17th at 10:15-12:15 (note this is 45 minutes earlier than the normal class period time)

Contact me

Department of Biology

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