Experimental

Procedure

 

Purpose: To determine the effectiveness of the meniscus once it has been freeze-dried and thawed out, and then undergone impact stress.
Hypothesis: The freeze-dried meniscus will be able to withstand the same amount of impact that the controlled meniscus can withstand, proving that the meniscus will be as functional as a normal one once allografted into the knee.
Materials:
*Two synthetic bones (these bones weigh about two pounds each, but are bone like in characteristic, like feel and texture).
*Two cow menisci, that are cut down to size to be fitted to the bone.
*Two pieces of string, one white and one green. These strings will be used to sew the meniscus to the bone.
*One needle, that will sew the menisci to the bone.
*One knife, used to cut the cartilage down to size.
*One microscope, to observe the results.
*One ten pound weight, one twenty pound weight, and one fifty pound weight.
*The stand that will hold the two bones in place during the impact.
*One digital camera to take pictures of the bones.
*One clock to regulate the time intervals.
*Dried ice and a cooler to put the meniscus in.
*One oven set at 98.7 degrees.
Procedure:
1. Take the two menisci and cut them with the knife into the same shape using a horseshoe piece of paper as the model.

2. Put one meniscus into the oven and the other into the cooler. Leave them both in their areas for three hours.

3. Take the frozen meniscus and put it into the oven for ten minutes.

4. Take the controlled meniscus and sew it in the bone.

5. Drop the femur bone with the ten pound weight first. Drop it four times. Then observe the results visually, and then under the microscope.

6. Take the now thawed one out and repeat step four and five.

7. Put both menisci back into their designated areas. Repeat steps 2-6, but using the twenty pounds instead.

8. Repeat all steps, but then using the fifty-pounds for the impact.

9. Record all results.