BEYOND THE BASICS:  FACILITATION OF ADVANCED TOPICS IN BIOCHEMISTRY

TABLE OF CONTENTS FOR LABORATORIES

Chemistry 464 Laboratory:   Principles of Biochemistry

1.    Safety Rules and Regulations
2.    Equipment List
3.    Biochemical Information: Library Assignment
4.    Buffers
5.    Peptide Analysis
6.    Spectrophotometric Determination of  b-Carotene
7.    Gel Filtration
8.    Glycogen Characterization  (by periodate cleavage)
9.    Chymotrypsin Catalysis
       (V_o vs  E_o; V_o vs pH)
10.  Lactate Dehydrogenase
       Isolation
       Purification on Affi-gel Blue
       Lowry Protein Assay
        (V_o vs  S_o+/- Inhibitor)
11.  Isolation of DNA
12.  Appendix
       How to Write a Scientific Paper (Robert A. Day)
       Guide to Report Writing in Journal Format
       Dos and Donts in Writing in Journal Format
       Affi-Gel Blue