BEYOND THE BASICS:  FACILITATION OF ADVANCED TOPICS IN BIOCHEMISTRY

TABLE OF CONTENTS FOR LABORATORIES

Chemistry 461 Laboratory:   Biochemistry I

1.     Biochemical Information:  Library Assignment
2.     Buffers
3.     Peptide Analysis
4.     Estimation of Apparent Molecular Weight by SDS-Gel Electrophoresis
5.     Characterization of Glycogen (by periodate)
6.     Spectrophotometric Analysis (Background)
7.     Chymotrypsin Catalysis
        (V_o vs  E_o; V_o vs pH)
8.    Lactate Dehydrogenase
       Isolation
       Purification on Affi-gel Blue
       Lowry Protein Assay
        (V_o vs  S_o+/- Inhibitor)
9.    Appendix
       How to Write a Scientific Paper (Robert A. Day)
       Guide to Report Writing in Journal Format
       Affi-Gel Blue