NSF IPI-IP sandra.jewett@csun.edu

BEYOND THE BASICS:  FACILITATION OF ADVANCED TOPICS IN BIOCHEMISTRY

TABLE OF CONTENTS FOR LABORATORIES

Chemistry 365 Laboratory:   Introduction to Biochemistry

1.    Biochemical Information:  Literature Assignment
2.    Biological Reactions - Existence of Enzymes
3.    Spectrophotometric Analysis:  Spectrophotometric Determination of  b-Carotene
4.    Protein Separations
5.    Isoelectric Point of Casein
6.    Paper Chromatography of Amino Acids
7.    Gel Filtration Chromatography
8.    Wheat Bran Phosphatase
       Preparation of a Crude Phosphatase
       Effect of Enzyme Concentration and Temperature on Reaction Rate
       Effect of Enzyme, Substrate and Inhibitor Concentration on Reaction Rate
       Journal Report Writing
9.    Preparation and Hydrolysis of Potato Starch
10.  Dialysis
11.  Extraction, Saponification and Identification of Nutmeg Triglyceride
12.  Enzymatic Hydrolysis of Triglycerides