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California State University Northridge Biology 470 - Biotechnology |
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Baculovirus expression system (Mirror)
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California State University Northridge Biology 470 - Biotechnology |
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Baculovirus expression system (Mirror)
This is a mirrored site. The original sites can be accessed at http://www.invitrogen.com/catalog_project/cat_baculo.html
and http://www.invitrogen.com/catalog_project/cat_bacvecs.html
pBlueBac4.5
pBlueBac4.5 is an upgrade of pBlueBac4. An ATG start codon, created by the presence
of an Nco I site in the multiple cloning site, has been removed by replacing the
Nco I site with an Xba I site and a Sma I site. pBlueBac4.5 is still a polyhedrin
promoter-based baculovirus transfer vector that has the following unique features:
| Small size - pBlueBac4.5 is only 4.9 kb. This is about half the size of most baculovirus transfer vectors. The small size of pBlueBac4.5 means cloning your gene of interest is faster and easier. Blue screening - pBlueBac4.5 coexpresses b-galacto sidase when cotransfected with Bac-N-Blue DNA* to produce recombinant plaques that are blue. This makes screening and selecting recombinant plaques easy. |  |
1. Recombinant
2. Wild type
credit: http://www.invitrogen.com/catalog_project/cat_bacvecs.html
Baculovirus Expression SystemMaxBac® 2.0 - Complete Baculovirus Expression System
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| The MaxBac 2.0 Kit contains everything you need to express your protein in the baculovirus
expression system. This updated version of our original MaxBac Kit contains our new
Bac-N-Blue AcMNPV DNA. The Bac-N-Blue DNA yields greater than 80% recombinant virus,
and because it can be used with any polyhedrin promoter-driven baculovirus expression
vector, it is the most versatile AcMNPV DNA available. For fast, simple identification
of recombinant plaques, the MaxBac 2.0 Kit contains the new pBlueBac4 vector. This
vector is incredibly small (only 4.8 kb) to make subcloning easy and coexpresses
the beta-galactosidase gene to produce blue recombinant plaques when cotransfected
with the Bac-N-Blue DNA. The MaxBac 2.0 Kit also contains our highly efficient Insectin
liposomes and your choice of frozen or log phase Sf9 cells. |
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credit: http://www.invitrogen.com/catalog_project/cat_baculo.html
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