Chapter 6: Manipulation of gene expression in prokaryotes

Supplemental material

Introductory material: Overall discussion of prokaryotic gene regulation from the MIT hypertextbook

• Operon model systems
• Fusion proteins
• Unidirectional tandem arrays of genes
• Translational control (Glick pp. 127-8)
• Protein stability
• Oxygen limitation (Glick pp. 129-130)
• DNA integration
• Secretion of protein products
• Control of metabolic load (Glick pp. 135-138)

Chapter 7: Recombinant protein production in eukaryotic cells

Supplemental material

• Yeast expression systems
  • Saccharomyces cerevisiae
  • Picchia pastoralis - mirrored image from Invitrogen
• Insect cells