University of Pittsburgh Site (continued)

http://www.pitt.edu/~rsup/mgbresupfac10.html

In the coupling reaction an activated intermediate is created by simultaneously adding the phosphoramidite nucleoside monomer and tetrazole, a weak acid, to the reaction column. The tetrazole protonates the nitrogen of the phosphoramidite, making it susceptible to nucleophilic attack. This intermediate is so reactive that addition is complete within 30 seconds. The phosphoramidite is blocked at the 5'-OH with the dimethoxytrityl group.

Please Scroll down

NEXT STEP

Back to DNA synthesis Page