http://www.pitt.edu/~rsup/mgbresupfac8.html
The phosphoramidite method of oligonucleotide synthesis is the chemistry of choice
for most labs because of efficient and rapid coupling and the stability of the starting
materials. The synthesis is performed with the growing DNA chain attached to a solid
support so that excess reagents which are in the liquid phase can be removed by filtration.
Therefore, no purification steps are required between cycles. The support material
is a form of silica or polystyrene beads. The particle size and pore size have been
optimized for liquid transfer and mechanical strength. The starting material is the
solid support derivatized with the nucleoside which will become the 3'-hydroxyl end
of the oligonucleotide. The nucleoside is bound to the solid support through a linker
attached at the 3'-hydroxyl. The 5'-hydroxyl is blocked with a dimethoxytrityl (DMT)
group.
NEXT STEP
Back to DNA synthesis Page