http://www.abrf.org/ABRFNews/1994/December1994/dec94ponoligo.html Tips For Oligonucleotide Synthesis Richard T. Pon - University of Calgary Oligonucleotide synthesis is by far the most common nucleic acid service provided by core facilities. The chemistry and instrumentation required has been extensively optimized, and excellent results can be obtained by almost anyone with a DNA synthesizer and good quality reagents. Indeed, the synthesis of short (less than 40 nucleotide) probes and primers has become so easy that no special expertise, other than the ability to read the operator's manual is required, and a part-time operator with a single or double column DNA synthesizer can handle the requirements of a small group. Click to read more...