The DNA Core Facility uses the ABI 394 DNA Synthsizer. This
is a four column instrument which means it can make four oligos together. This DNA
synthsizer makes excellent oligos and the cost of oligos is relatively low. This
is one benefit that can be passed to the end user. Turnover time is usually 1 to
2 days depending on the work load
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 Reverse compliment any sequence with
this form
NEW Use this
form to search the DNA
Core's Oligo Database
Use this Oligo Request Form to order oligos over the World Wide Web.
Use this form to calculate oligo melting temperatures.
Use this form to reverse complement your sequence or oligos.
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All oligos are made using a 40 nano mole polystyrene column,
unless otherwise requested. Polystyrene is very hydrophobic and consequently makes
a better oligo than the old CPG (Control Pore Glass) columns. That means N - 1 products
are kept to a minimum, especially when making sequencing or PCR primers. Read about
the steps involved in synthsizing oligos on ABI instruments. Oligos synthesized
for PCR or sequencing can be purified very easily without the need for columns or gels.
All oligos come ready to use, in water.
The Core Facility can also synthesize modified oligos such as degenerate oligos,
biotin labeled oligos and phosphorothioate oligos for anti-sense work.
Oligos Will Not Be Synthesized Without a Grant
No. for Joslin Users or PO No. for All Others.
The cost of oligos is: |
