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Innoculation of Legumes

Guide A-130

W.C. Lindemann, Soil Microbiologist
C.R. Glover, Extension Agronomist


Nitrogen fixation in legumes depends on the formation of nodules by Rhizobium. Without sufficient nodule mass filled with an efficient, nitrogen-fixing strain of Rhizobium, nitrogen fixation will be inadequate. Inoculation of legume seed assures Rhizobium is present in the root environment.

Rhizobium is a common soil bacterium. Rhizobium is not toxic to humans, plants, or animals. It is one of the most beneficial bacteria to agriculture. Some Rhizobium are specific and nodulate only specific legumes, while others may nodulate several legumes. Native Rhizobium may be in sufficient numbers to nodulate both native and introduced legumes. Often, the native Rhizobium are low in numbers, are the wrong species or strain for the introduced legume, or are not efficient nitrogen fixers. Inoculation usually corrects these problems.


Growers must decide if they need to inoculate before planting. The following considerations should help decide if inoculation is necessary.

1. If the legume to be planted was never grown or hasn't been grown in the field for more than 5 years; if the legume was grown and you don't know if it was well nodulated or how efficient nitrogen fixation was; or if the land was never in cultivation (native desert or range), it is wise to inoculate.

The native Rhizobium is probably not in sufficient numbers to adequately nodulate the crop or may not be efficient in fixing nitrogen. Inoculation is inexpensive (less than $5 per acre) compared to the expense of replanting. An exception is when surrounding fields are planted or have been planted to the same legume that are well nodulated. Rhizobium will spread to nearby fields after years of legume production. Alfalfa grown in the Mesilla Valley is a good example.

2. If the legume was previously grown, but nodulation or nitrogen fixation was poor (as indicated by poor yield and nitrogen deficiency symptoms), the grower should inoculate. A grower should always determine the cause of a poor yield (insect, disease, weather, water, plant nutrition problems). If the field was inoculated and poor nodulation or poor nitrogen fixation resulted, elimination of plant stresses may also increase nodulation and nitrogen fixation.

3. If the legume was previously grown, was well nodulated and nitrogen fixation appeared adequate (as indicated by good yield and no nitrogen deficiency symptoms), there is probably no need to inoculate. Once Rhizobium is established in a field, it persists for several years between crops. There is no yield advantage to yearly inoculation of legume crops in fields where the well nodulated legume is routinely grown. Also, once a particular strain of Rhizobium is established in a field, it is difficult for another strain to establish itself. The established strain is usually more competitive than an introduced strain for nodulation sites on the plant.


Several inoculate types are available. These include powder, granular, liquid and frozen concentrate. Pre-inoculated legume seed may also be available. The powder and granular inoculates are the most common, with liquid and frozen concentrate inoculates used for special purposes, and generally not recommended for routine inoculation problems. Liquid inoculates are only useful for a few days after they are produced, and are not recommended.

Powder inoculates are the most common and most reliable. Powder inoculates have been around since the turn of the century and are simply stuck to the seed before planting. Powder inoculates are available for all types of legumes, and are the least expensive to use (less than $5 per acre). The black, or occasionally tan, powder contains approximately one billion Rhizobium per gram.

Granular inoculates are available for large-seeded legumes including beans, soybeans and peanuts. They contain about the same concentration of Rhizobium as the powder inoculate. Particle size is larger than the powder, which allows the inoculate to flow through an insecticide, fertilizer or similar planter attachment. Although the cost may be higher, the inoculate can be distributed by the planter attachment and no seed mixing is required. Granular inoculate is particularly useful when pesticide-coated seed is planted, or when excessive seed handling can damage the seed coat, as with peanuts.

Pre-inoculated seed is worth the extra price paid for it (as compared to uninoculated seed) only if the inoculate was freshly added and the seed quickly planted. This is not generally the case in New Mexico. Rhizobium is a living organism and will not survive long if left exposed to air, heat, cold or light if inoculation is considered necessary, use the previously discussed methods and don't waste money on pre-inoculated seed.

Several inoculate companies produce legume inoculates, but only two serve New Mexico. They sell only to agricultural suppliers, and then only in case amounts. However, they can be contacted directly and may supply a grower or supplier when asked. They sell inoculates under several brand names. The two suppliers that serve New Mexico are:

Research Inoculates
310 S. Third Street
St. Joseph, MO 64501

Nitragin Inc.
PO Box 09186
3101 W. Custer Avenue
Milwaukee, WI 53209

For home gardeners, many garden seed companies offer small quantities of powder inoculates for a variety of garden legumes. These are usually called garden packs, and contain several types of Rhizobium for most commonly grown garden legumes.


Rhizobium inoculate is a living culture of bacteria and must be handled as such. Rhizobium are particularly sensitive to heat, desiccation and light. Many inoculate failures can be traced to improper purchasing and handling of the inoculant. If the inoculate is handled as a fertilizer or pesticide, either by the grower or supplier, the Rhizobium will likely be dead before it reaches the seed. Inoculant should be stored in a cool area away from desiccation and heat and refrigerated if it is to be stored for some time. Pesticides and some fertilizer coating may kill Rhizobium when applied directly to the seed. Always make sure the inoculant label contains the following critical information: type of legume, quantity of seed (powder) or acreage of land (granular) the package will inoculate, and expiration date.

The Rhizobium species must match the legume species. Alfalfa inoculate will not work on beans and bean inoculate will not work on alfalfa. Powder legume inoculates should indicate how many pounds or bushels of seed the inoculate should cover/inoculate. Never use less than the recommended amount of inoculate for the given quantity of seed. If conditions are particularly hot or dry when planting, the inoculate is old or has been stored improperly, or the land has never been cultivated with legumes, double the amount of inoculate per quantity of seed. Adding double, triple, or 10 times the recommended rate will not affect the environment or seed. Granular inoculates are labeled to indicate how many acres the inoculate will cover. Adding more than the recommended rate is much more costly with granular than powder inoculates.

The expiration date is critical because inoculates are living cultures and their viability decreases with time. Never buy an inoculate that has passed the expiration date. Never buy an inoculate that has been stored improperly by the supplier, such as in a chemical or fertilizer warehouse.


When inoculating with a granular inoculate, simply follow the label instructions. Never allow the inoculate to remain in a planting attachment in the hot sun for long. Remove unused inoculate from the attachment, seal it in the original bag, and store in an area protected from heat and sun.

Powder inoculates require more handling. They must be securely stuck to the seed, and the seed planted immediately. Commercial stickers are available that help stick the inoculate to the seed. You can make your own sticker by preparing a sugar solution of 1 cup sugar to 1 quart of warm water. Slightly moisten the seeds with a small amount of water or sticker, and mix with inoculate. Only a small amount of liquid should be used to prevent the seeds from getting too wet. A small cement mixer mixes the seed and inoculate uniformly. Pouring seeds and inoculate between clean 5-gallon buckets also works well. After mixing, all the seeds should be evenly covered with small specks of inoculate. Plant the seeds immediately, making sure the inoculated seeds are not exposed to sunlight. If the seeds become too dry, the inoculate will come off. If large quantities of seed are to be inoculated and stored for several hours or overnight, put the inoculated seeds back in the original seed bag, add a moistened paper towel, and close tightly. Inoculate poured on the top of seed in a planter will result in poor inoculation.

The information given herein is supplied with the understanding that no discrimination is intended and no endorsement by the Cooperative Extension Service or New Mexico State University is implied.

New Mexico State University is an equal opportunity/affimative action employer and educator. NMSU and the U.S. Department of Agriculture cooperating.

Written: May 1990
Placed on Server: March 22, 1996

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