DNA Sequencing Facility

The Facility:

• Sequencing of PCR products, plasmid, phage and lambda DNA. We utilize ABI Prism^tm 377 DNA Sequencer platform with BigDye^tm  v.3.1 chemistry.
• The sequences can be obtained using custom primers (provided by clients) or our standard primers (provided at no extra charge).
• pGEM M13 sequencing is used as a positive control with every run.

 
• Custom primer design for sequence walking.

 
• Sequence assembly.

How many nucleotides can be read from one reaction?

• Sequence lengths of greater than 650 bp are expected from high quality DNA templates.
• The length of the DNA sequence is highly dependent on the quality and accurate quantification of the template.

Cost of Services

• For educational institutions basic sequencing is provided at a cost of $15 per reaction and $25 per reaction for both a miniprep and sequence. Industrial prices are higher.
• Bulk rates apply.

Turn around time is usually 2-3 days. If the time is greater, you will be notified.
 
 
 

Sample Preparation and Shipment

Plasmid/Lambda/Phage Samples:

• The templates can be sent either lyophylized (dry) or in WATER only (no salts please) on ice.
• A quantity of 0.5 ug per reaction is required at a concentration of 0.2 ug/ulor greater.
• Please include the type of vector, the size of the insert and an accurate concentration.

PCR Samples:

• The templates can be sent either lyophylized (dry) or in WATER only (no salts please) on ice.
• A quantity of 20 ng per 100 bases in length of template at a concentration of 20 ng/ul or greater.
• Please state that template is a PCR product, the length of the template and an accurate concentration.
• Gel diagram is helpful.

Primers:

• Sequencing can be obtained with either your custom primers or our standard primers
• Primers must be in water at a concentration of 1pmol/ul or greater or dry with known molar value.
• Please include the concentration of your primers.
• We can provide most standard primers for sequencing vectors (M13, T7, T3, SP6, SK, KS).

Shipping:

• Samples can be mailed directly to the CSU Northridge DNA Sequencing Facility (see contact information).
• Samples can be sent by standard mail or through a delivery service (UPS, FedEx, etc.) depending on your needs.
• Be sure to pack your samples carefully!
• Do not send samples in single thickness envelopes - the US Postal Service is VERY hard on mail.
• A padded envelope, or a box is recommended as well as wrapping your tubes in Parafilm.

Data

• Your sequence data can be transferred electronically (both text and or electropherograms).
• If you do not have software for interpreting electropherogram files, a free version of EditView (for Mac platform) can be downloaded from the Apllied Biosystems website (download starts automatically)   PC platform based software packages include Chromas, GeneTool and others.
• Hard copies of the electropherograms will be mailed upon your request.
• If you wish a hard copy of the text and/or electropherogram data files please include a zip disk.

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