PROFESSOR MARY LEE SPARLING
Prerequisites :cell biology and genetics or equivalent-see instructor
The course will emphasize
integration of cross-disciplinary knowledge of cell control mechanisms
the ligands and receptors and ion channels and
tranduction mechanisms involved in control of normal cell
polarity, cell communication, metabolism, motility, circulation, development,
reproduction, cancer and disease and the drugs which can effect the control.
Protein structure-function relationships and evolutionary changes allowing
functional change or specialization.
Cytoskeletal and motor components utilized to polarize
cells, their mRNA, their membrane receptors and cytoplasmic organelles.
Control of secretion, organelle transport.
Control of chromatin, genes, transcription,
translation involved in changing or maintaining cell behavior, or finely tuning
gene products for dealing with environmental challenge, adaptation.
THE
COURSE WILL HAVE A TEXTBOOK: Molecular Biology of the cell,
fourth edition: Alberts, Johnson, Lewis, Raff, Roberts, Walter. 2002, and the
supplementary text: Medical Physiology,A cellular and Molecular approach WF
Boron and EL Boulpaep, 2003
Course content outline
FOR LISTS OF SLIDES USED IN
LECTURE
Syllabus for lecture
(tentative)
BIOLOGY 580 LAB Lab experiments: we will study normal activities and compare to drug
treated, to ind out what inhibits or activates processes. Lab reports each
week. Standard curves, use of instruments. Chick: cell dissociation and
reaggregation, Tissue culture, muscle contraction and antibody stain, Kidney tubule transport and
function Fish: partial purification
of actin and myosin, Anti-myosin stain,
Myosin ATPase and enzyme kinetics, Scale pigment contraction Frog: acetylcholinesterase-
heart and muscle, Mouth and oviduct cilia, Egg- fertilization and cleavage, Embryo tissue culture. Sea urchin: fertilization,
artificial activation, Osmotic properties of eggs, Motility, properties of sperm, Endocytosis, Phospholipases,
TLC Elodea: protoplasmic
streaming Paramecium : ciliary
control, contractile vacuole Euglena- motility,
phototropism Lily : stomata opening
If student interest is there, we can use microelectrodes to Study membrane potentials-requires computerese
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HOW
CAN CELLS STABILIZE OR CHANGE BEHAVIOR?
ALTERING CYTOSKELETON- three lectures
REGULATE ASSEMBLY
REGULATE ASSOCIATIONS
ACTIVATION OF MOTORS
MEMBRANE TRAFFIC
CELL MOTILITY AND SHAPE CHANGE- three lectures
SETTING CELL POLARITY-three lectures
CHANGING GENE ACTIVITIES- three lectures
ALTERING CHEMICAL EQUILIBRIUM- two lectures
SWITCHING METABOLISM- three lectures
MEMBRANES AND CELL ADHESION
CHANGE RATE OF GROWTH AND CELL DIVISION
RESET BIOLOGICAL CLOCKS
CELL COMMUNICATION
CELL SIGNALS-neurotransmitters, hormones
CELL RECEPTORS
MEMBRANE CHANNELS
SIGNAL TRANSDUCTION
PHOSPHORYLATION
UBIQUITINATION
RELEASE OF CALCIUM
O2 SUPPLY, HEMOGLOBIN AND CIRCULATION
NITROGEN, SALT, WATER AND KIDNEYS