LABORATORY SCHEDULE 2006
SPRING- SPARLING BIOL 441L print
out each experiment the week before there are done from the lab manual from the
web at www/csun.edu/~vcbio001. Be sure to check out a book from the library
or reserve book room every
week to help you with the lab. Just one textbook and one atlas is not
enough to learn the lab material. You need lots of description and picturesl
Your laptop computer will come in handy in lab, as will a digital camera, if
you have them, or a scanner. The lowest grade for one lab will be dropped, so everyone can miss
onelab, but after that, the grade will be decreased for each lab missed. KEEP
YOUR CORRECTED EXPERIMENTS IN A LAB NOTEBOOK SINCE IT MUST BE TURNED IN AT THE
END OF THE SEMESTER. You can share lab data only with your lab partners, plot
data and write up experiments separately. Do not loan your lab book to anyone.
If WRITEUPS ARE TOO MUCH ALIKE, POINTS WILL BE DEDUCTED, AND IT WILL BE CONSIDERED
CHEATING. If a lab partner is absent do not share data with them. +,- GRADING
WILL BE USED.
|
Date |
Topic |
Assignment- print out the lab manual at the web page
with sheets to be handed in each day |
TEXTBOOK: :required Gilbert 7th edition;
Schoenwolf and Matthews p., Sparling lab manual |
|
Jan 30 |
Get books, hand out slides, start exercise 1, discuss
how to find information |
|
|
|
Feb 1 |
MEIOSIS,OVULATION exercise #1 from Sparling
lab exercises
|
FIND ALL UNDERLINED STRUCTURES, hand in lab sheet at
end of lab-10 points |
Chap 19, M&S p 7-9 |
|
Feb 6 |
MAMMAL TESTIS,SPERMATOGENESIS, Ascaris FERTILIZATION and
maturation Exercise #2 HORMONES |
|
M&S 2-6; 17-21 |
|
Feb 8 |
: Sea urchin fertilization ; Animalization and
vegetalization experiments |
Learn steps in early development ;find diagrams of
components, relate this to what you
see in your experiments |
Hand in lab experiment writeup within a week-10
points. |
|
13 |
phospholipase TLC or starfish exp, learn ImageJ-
download from NIH |
Makes tables or lists showing genes, what controls
them and what structures or processes they control |
Hand in lab experiment writeup within a week-10
points |
|
15 |
Early frog
development-CLEAVAGE AND SETTING AXES
IDENTIFY AND DIAGRAM GENES INVOLVED IN CELL AND EMBRYO POLARITY, AXES |
Show instructor required items in your slides. |
Look at factors causing A-P, D-V, M-L axes formation |
|
17 |
|
|
LAST DAY TO add,
DROP WITH NO APPROVAL |
|
20 |
4 MM FROG |
Show instructor required items in your slides,
use computer to test yourself, learn landmarks of demarcation |
Turn in your list of found structures initialed by
instructor-10 points |
|
22 |
7 MM FROG |
|
Turn in your list of found structures initialed by
instructor-10 points |
|
27 |
7 MM FROG |
Show instructor
required items in your slides |
Turn in your list of found structures initialed by
instructor-10 points |
|
Mar1 |
MEIOSIS,FERTILIZATION,GENES OF AXES SETTING, frog TEST, 2ND HOUR 100 points |
20microscopes with three items on each one to
identify |
|
|
6 |
Xenopus experiment-Write up your data and results,
discuss the results in terms of mechanisms involved in those results |
|
Hand in lab experiment writeup within a week-10
points |
|
8 |
Regeneration experiments on
tadpole tails, removal of eye or gill region Write up your data and results,
discuss the results in terms of mechanisms involved in those results |
|
Hand in lab experiment writeup within a week-10
points |
|
13 |
Look at 13,18,24 hr chicken slides. MAKE LISTS AND DIAGRAMS OF RECEPTORS, SCAFFOLDS,
ENZYMES, CYTOSKEL CONNECTIONS OF SIGNALING for cleavage, gastrulation,
axiation |
Show instructor required items in your slides |
Compare axes formation in chicks with that of frog- Turn
in your list initialed by instructor-10 points |
|
15 |
48 HR CHICK, |
Show instructor required items in your slides |
Turn in your list of found structures initialed by
instructor-10 points |
|
20 |
48-72 HR CHICK, |
Show instructor required items in your slides |
Turn in your list of found structures initialed by
instructor-10 points |
|
22 |
72 hr |
Show instructor required
items in your slides |
Turn in your list of found structures initialed by
instructor-10 points |
|
25 |
72 hr , 96 HR CHICK |
Show instructor required
items in your slides |
Turn in your list of found structures initialed by
instructor-10 points |
|
27 |
LIVE CHICK EGGS heart rates in different
temperatures, compare extraembryonic membranes of several stages . Write up
your data and results, discuss the results in terms of mechanisms involved in
those results |
Read about possible experiments in lab manual and
text before class |
Hand in lab experiment writeup within a week-10
points |
|
29 |
LIVE CHICK EGGS cell dissociation-adhesion. Write
up your data and results, discuss the results in terms of mechanisms involved
in those results |
|
Hand in lab experiment writeup within a week-10
points |
|
April 5 |
Chick test |
20microscopes with three items on each one to
identify |
|
|
17 |
Frog experiments |
Read about possible
experiments in lab manual and text before |
Hand in lab experiment
writeup within a week-10 points |
|
19 |
,Teratogenesis- abnormal
development |
Read about possible experiments in lab manual and
text before class |
|
|
24 |
. Draw or photograph results. Antibody
immunohistochemistry on cultures- hand in questions |
Read about possible experiments in lab manual and
text before class |
|
|
26 |
6 and 10 mm pig slides and little pigs |
|
|
|
May 1 |
Digestive system |
|
GENE CONTROL digestive SYSTEM MAKEDIAGRAMS Turn
in your list of found structures and diagram initialed by instructor-10
points |
|
3 |
Heart and veins |
|
|
|
8 |
Arteries |
|
Turn in your list of found structures and diagram initialed
by instructor-10 points |
|
9 |
urogenital |
|
|
|
15 |
Changes at birth |
|
|
|
17 |
Pig test and hand in all experiments not yet graded |
|
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This schedule is only an
outline because we may have to change the order of labs due to availability of
materials